Deciphering the molecular organization of the cell wall, capsule and extracellular vesicles of Cryptococcus neoformans by innovative solid-state NMR methods.
Context : We propose to develop innovative solid-state NMR techniques to uncover the molecular organization of polysaccharides and proteins of the cell envelope of C. neoformans. The project is funded by the ANR.
Laboratory: The project will take place at the IECB (Institut Européen de Chimie et Biologie) in the team of Antoine Loquet.
Selected publications of the team: Solid-state NMR molecular snapshots of Aspergillus fumigatus cell wall architecture during a conidial morphotype transition. Lamon G, Lends A, Valsecchi I, Wong SSW, Duprès V, Lafont F, Tolchard J, Schmitt C, Mallet A, Grélard A, Morvan E, Dufourc EJ, Habenstein B, Guijarro JI, Aimanianda V, Loquet A. Proc Natl Acad Sci U S A. 2023 Structural and molecular basis of cross-seeding barriers in amyloids. Daskalov A, Martinez D, Coustou V, El Mammeri N, Berbon M, Andreas LB, Bardiaux B, Stanek J, Noubhani A, Kauffmann B, Wall JS, Pintacuda G, Saupe SJ, Habenstein B, Loquet A. Proc Natl Acad Sci U S A. 2021 Magic-angle spinning NMR spectral editing of polysaccharides in whole cells using the DREAM scheme. Delcourte L, Berbon M, Rodriguez M, Subban K, Lends A, Grélard A, Morvan E, Habenstein B, Saupe SJ, Delhaes L, Aimanianda V, Daskalov A, Loquet A. Methods. 2024
